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Samples collected at 2 h and 1, 6 and 32 DAT were used to measure fruit lignin content.
So, samples from 2 h after treatment and 1, 6 and 32 DAT were used to detect lignin content changes over time.
Samples collected at 2 h and 1, 6 and 32 DAT were used to measure hydrogen peroxide levels based on the methods of Patrick and Wagner [ 45] with some modifications.
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However, CE-DAT was used to corroborate the Uppsala data and there were no significant differences.
Initially, single-pinhole SPECT systems were used to image DAT [ 91, 100], and the SPECT images were coaligned with MR images (or templates) acquired on clinical MRI scanners (using dedicated coils), with or without the use of external markers [ 99, 101, 102].
A SIP 95 sound level meter, a Symphony (01dB Stell©), and a DAT FOSTEX PD-4 (44.1 kHz sampling frequency) were used to record the acoustic data.
Samples collected at 1, 6, 12 and 32 DAT were used for enzyme activity determination.
Samples at 1, 6, 12 and 32 DAT were used for differential analysis of proteins accumulation.
Samples collected at 2 h and 1, 6 and 32 DAT were used for the determination of fruit firmness.
Samples collected at 2 h and 1, 6 and 32 DAT were used for the differential primary metabolic profiling analysis.
Samples at 2 h and 1, 6 and 32 DAT were used for the differential secondary metabolic profiling analysis using the HPLC-QTOF-MS instrument.
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