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Rough EmerAnds.
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This indicates that the diameters of varicosities and intervaricose segments in axons with DAT-Type I, DAT-Type II, and DAT-Type III labeling patterns are similar and that the measurements made in cross-section probably contained a larger number of varicosities for axons with DAT-Type II and DAT-Type III patterns than for axons with DAT-Type I labeling pattern.
We name this pattern of labeling DAT-Type II (Fig. 5 B ). Overall, the immunoprecipitate was paler in axons with DAT-Type II than in those with DAT-Type I immunolabeling.
Finally, 2 axons with DAT-Type I immunolabeling and one with DAT-Type II immunolabeling established appositional contacts without membrane specializations, classified as puncta adherens.
DAT-Type I and DAT-Type II labeling patterns were present in 62% and 20%, respectively, of the studied axon population.
Most contacts (n = 7) occurred in axons with DAT-Type I immunolabeling.
We name this pattern of labeling DAT-Type I (Fig. 5 A ).
We name this pattern of labeling DAT-Type III (Fig. 5 C ).
Bona fide synaptic junctions with clear membrane specializations were observed in 5 axons with DAT-Type I immunolabeling and in one axon with DAT-Type II immunolabeling; 5 of these synaptic junctions were asymmetric (4 in axons with DAT-Type I immunolabeling, one in the axon with the DAT-Type II immunolabeling) and one was a symmetric contact (in an axon with DAT-Type I immunolabeling).
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