JC and AKi performed the DArT marker discovery analysis, developed the DArT array and genotyped the two mapping populations with the DArT markers.
DArT marker names are standardised and automatically generated by a DArT-specific Laboratory Information Management System (DArTdb; DArT P/L, Canberra, Australia).
DArT marker names are standardized and automatically generated by a DArT-specific Laboratory Information Management System (DArTdb; DArT P/L, Canberra, Australia).
Garovaglia DArT marker presence was distributed as follows: G. powellii private (30%), G. elegans ssp.
For the amplicons derived from the DArT marker sequences, the DArT fragments CABMR_07D07 (129 bp; LG1) or AHMR_08O09 (728 bp; LG15) served as positive PCR controls.
viride shared marker class included 6 DArT markers absent from global limestone and 1 DArT marker absent from serpentine (marker ID Asplenium3O24).
Importantly, DArT procedures hold the potential to generate co-dominant markers by taking into account the strength of the signal for each DArT marker.
To detect DArT marker specificity by substrate or geography, we examined the distribution of DArT markers in substrate-specific or geographically defined Asplenium and Garovaglia specimens.
The Asplenium DArT marker data set suggests that serpentine was the ancestral substrate of A. viride with two independent colonizations onto limestone (Figure 3).
The in vivo genomic copy number of each DArT marker was approximated by hybridizing un-amplified metagenomic DNA to each genotyping array.
PD contributed to DArT marker development.
