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Samples were incubated with primary antibodies 60 min at 4 °C in dark, step followed by three washes with PBS.
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Suddenly something large and dark stepped out ahead of him.
dark steps across this pale grass perfect with dew, dark steps so early, so swift, the short length of this long lawn... From How Snow Falls, by Craig Raine (Atlantic, £14.99).
A brief visit down the dark steps to the underground prison revealed rows of small, barred cells, the air close with smoke from a recent American airstrike.
Vito is a family man and a businessman, blessed to be in America, but led astray and terribly pained to think that his most beloved son, Michael, might have to follow his own dark steps.
This time round we follow Hot Creations' North Eastern general Richy Ahmed down the dark steps of the past.
I didn't want to wake anyone up so I clambered into the top bunk in the dark, stepping on someone's face in the process, and slept fully-clothed, hugging the camera, and crying.
In the present study a mixture of starch and glucose was used as carbon source for the dark fermentation step.
But the applicability as an online sensor depends on the development of an automatic dark adaption step.
If a dark adaption step can be established in a routine measurement the change of pigment composition within a species over time has to be considered.
However, because the cell-type specific transcript level of photoreceptors depends on the developmental stages, we analyzed their transcript levels right after separation of cell types, i.e. three hours before initiation of cleavage division, and at the end of dark incubation step (Additional file 1: Figure S1A-B).
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