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The resulting strains (LtZ1-4) increastromulemule frequency, stromule length and dark starch staining reminiscent of GV and GVR infiltrations.
Although we infiltrated in a restricted region of the leaf (outlined in Figure 5C and 5D with dotted lines) dark starch staining was not limited to the infiltrated region, but spread to adjacent tissue, implying the spread of the hormone.
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The results indicated that the C. moewusii SAG 24.91 strain had faster rates of dark anaerobic starch catabolism than C. reinhardtii, with different profiles of fermentation end-products.
This well established and sensitive method relies on the oxidation of iodide to molecular iodine (oxidizer +2I− → I2+ red. oxidizer) and subsequent formation of I3− ions (I−+I2 → I3−), that form a highly-colored complex with starch (dark blue areas in Fig. 5).
Experimentally, if the onset of darkness unexpectedly occurs only 6 hr after the previous dawn in plants previously grown in 12-hr light/12-hr dark conditions, the starch is degraded too quickly, while for an unexpected onset of darkness at 8 hr, the starch degradation occurs at the appropriate rate (Lu et al., 2005; Graf et al., 2010).
High quality DNA was obtained as follows: the unfolded tender leaves of N. nucifera were harvested and stored at 4°C in the dark to eliminate starch from the tissue.
DNA was isolated from 100 g of expanding leaves of Heinz 1706 seedlings harvested 21 d after planting, with the final 3 d of growth in the dark to reduce starch content.
Biohydrogen can be produced through dark fermentation using sugars, starch, or cellulosic materials.
The effects of Fe0 and Ni0 nanoparticles on mesophilic dark hydrogen fermentation from starch were investigated using heat-shock pretreated anaerobic sludge in batch reactors.
Wild type cells containing starch stained dark purple when exposed to iodine vapour.
Results for glucan, water dikinases and α-amylases affecting "dark" region of the starch pattern are accordant with their proposed functionality.
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