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Showing dark staining of the nucleus, indicating increased chromatin content.
Cells that show a prominently dark staining also were increased in size and had the appearance of cells migrating through the basement membrane (Fig. 2c, black arrow).
The image (Fig. 1A) showed the dark staining surrounded the bright entities, indicating the presence of spherical particulates, and the NPs were uniform in size.
Complete dark staining of pollen with (I2KI) solution was considered as viable.
Dark staining deposits were found only in two species (Cosmos parviflorus and D. papposa).
Although we did not perform histochemical tests for tannins, the dark staining deposits that we described may be these contents.
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The chalazal megaspore continued to enlarge and served as the functional megaspore, while the micropylar megaspore had compressed and become a thin dark-staining layer (Figs. 4f, 5f).
At 18 h, the cell contains multiple dark-staining digestive vacuoles and swollen mitochondria.
Additionally, there are some dark-staining deposits, which could be flavonoids or alkaloids, in some of the epidermal cells on the adaxial surface.
Argyreia breviscapa, A. kerrii, and A. suddeeana have dark-staining deposits in some of the cells on the adaxial surface (Fig. 5a c).
a, g A. breviscapa, b, h A. kerrii, c, i A. suddeeana, d, j A. variabilis, e, k Argyreia 1 and f, l Argyreia 2 (ds dark-staining deposit, ep epidermal cell, pc palisade cell, sc spongy cell, tr trichome, vt vascular tissue).
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