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An example of this method is the drinking-in-the-dark procedure, which generally is regarded as a model of binge drinking because animals will consume an intoxicating dose in a relatively short time period (Rhodes et al. 2005).
KO and wild-type (WT) mice of both sexes were tested for intake of 20% ethanol, 10% sugar water and plain tap water using established drinking-in-the-dark procedures.
A light:dark box procedure and an elevated plus-maze procedure was used to measure anxiety-like behaviors and general motor activity.
The illumination followed by dark incubation procedure was repeated three times.
Therefore, BDE47 probably increased the photosensitive ability of zebrafish larvae and shortened their dark adaptation procedure, resulting in lack of responsiveness to changed light conditions.
Three animal models of anxiety were used: the elevated plus-maze, the light-dark test procedure and the free exploratory paradigm.
The entire dark-adapted procedure was carried out under dim red light (λ > 650 nm).
A live dead assay was then incubated for 15min in the dark before microscopic procedure using Zeiss "Axiovert S 100" and filter set #09 (Carl Zeiss AG, Jena, Germany).
After perfusion, the cerebella were placed into freshly made 2.5 % potassium dichromate (4-days) and 0.75 % silver nitrate 3-dayss) solutions at room temperature in the dark (Golgi staining procedure) (Friedland et al. 2006; Golgi 1995).
A 2002 study published in The Annals of Internal Medicine of one such incident found that the patient, doctors and nurses went along with the mistaken treatment because they were used to being kept in the dark about medical procedures.
Procedure for dark count maps (left), and for source count maps (right).
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