Sentence examples for dark mounted from inspiring English sources

Immunostained cells were then allowed to air dry in the dark, mounted in SlowFade Gold antifade reagent (Invitrogen), and examined with an Olympus IX81 microscope.

After the last incubation, cells were washed five times, dried in the dark, mounted with Vectashield medium (Vector Laboratories, Inc. Burlingame, CA) and cover glasses and analyzed using an inverted fluorescence microscope.

The cells were then washed with cold PBS, fixed in acetone/ethanol 70% (1 1) for 10 minutes at 4°C, stained with 49,6-dianidino-2-phenylindole 49,6-dianidino-2-phenylindole 49,6-dianidino-2-phenylindole 49,6-dianidino-2-phenylindole(dihydrochlorideGermany), anDAPIserved by fluorescence microscopy (Olympus, BX-UCB, Center Valley, PA).

Slides were washed three times with PBST, one time with PBS, stained with DAPI (1 5,000) for 1 h at 4 °C in the dark, mounted with coverslips, and imaged on a Zeiss LSM700 Meta Inverted laser scanning confocal microscope and a Leica DMI 6000B Inverted fluorescence microscope.

A "Rickshaw Obscura" allows visitors to climb inside a dark chamber mounted on a bicycle cart to see an upside-down outdoor vista projected against a wall; the image, produced by a pinhole, demonstrates the workings of a classic camera obscura.

After washing with ddH2O and ethanol, slides were dried in the dark and mounted with Moviol (Hoechst, Frankfurt, Germany).

After washing with PBS 3 times for 3 min, the sections were air-dried in the dark and mounted with citifluor.

After dehydration in EtOH, slides were air-dried in the dark and mounted in Vectashield/DAPI (3 parts Vectashield/DAPI + 1 part Vectashield, all vector).

The slides were air-dried in the dark and mounted in Duolink in situ Mounting Medium with DAPI (Olink Biosciences) and incubated at 4 °C overnight to allow DAPI to stain the chromosomes.

To detect caspase-8 protein expression immunofluorescence analysis was performed with cleaved caspase-8 antibody (#9496, Cell Signaling) followed by incubation with anti-goat IgG-FITC (Santa Cruz) for 30 min in dark and mounted with DAPI mounting medium (Vector Labs).

Coverslips were rinsed and allowed to air dry in the dark before mounting.