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Furthermore, the dark development phenotypes of hsr8-1 were not dependent on jasmonate responses [ 23].
Neither the phyA-201 [ 21] nor the phyB-1 [ 22] mutations suppressed the hsr8-1 developmentphenotypenotype (Fig. 4a).
The suppression of hsr8-1 developmentphenotypenotype in the soh715hsr8-1 mutant is therefore caused by the deletion of At1g25540, encoding the MED25/PFT1 protein [ 16, 17].
For dark development experiments, seeds were grown on MS medium containing 1 % glucose, exposed to light for 8 h and then grown vertically in complete darkness for 2 weeks.
We therefore assessed the extent to which PFT1 controls gene expression in response to glucose in light grown seedlings, and also how it controls gene expression during dark development in Col and hsr8-1 genetic backgrounds.
We concluded that PFT1-mediated suppression of reduced hypocotyl elongation in hsr8-1 was not dependent on PFT1 functioning as part of phytochrome- and JA- mediated responses, suggesting PFT1 functions through a independent mechanism(s) to reduce hypocotyl cell elongation during dark development of arabinose-deficient mutants.
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A darker development is the abuse of blogs, social networks, maps and video-sharing sites that make it easy to publish incendiary material and form hate groups.
Figure 4 shows that the dark-development phenotypes of hsr8-1 were not dependent on phyA or PhyB, and hsr8-1 did not significantly influence white- and far- red light responses.
From each of the enriched flasks, 1.5 ml of culture was taken in centrifuge tube and centrifuged for 5 min at 8000 rpm, 1 ml of supernatant was taken and mixed with 3 ml of Salkowski's reagent and incubated for 30 min in dark for development of pink color.
After a 10 min incubation at 37°C in the dark, color development (absorbance) was recorded at 405 nm using an automated ELISA plate reader (model EL311SX; Biotek, Winooski, VT).
Among the different transgenic types, the amiRPPO1 and amiRPPO3 series lines showed relatively stronger browning phenotype, followed by the amiRPPO23, amiRPPO234A and amiRPPO1234 series lines, based on the degree of dark color development that ranged from high to low.
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