However, the dark control was not significantly different from the dark treatments containing plastics (Fig. 3d).
This significantly enhanced photocytotoxicity relative to the dark control is an essential property of photochemotherapeutic agents.
Each transect was randomly assigned to use either white, red, or green LED lighting or to be a dark "control" treatment.
PyOM C mineralization was measured for both the photochemically weathered (i.e. UV treated PyOM or PyOMUV) and PyOM not exposed to high-energy light (i.e. PyOMW serving as a dark control).
The optimum environmental and nutritional conditions for arthroconidial germination in the dark control were estimated as follows: temperature, 26 °C; glucose, 20 g/L; soybean peptone, 10 g/L; Mg2+ concentration, 2 mmol/L; and initial pH, 3.5.
A full factorial design of 240 experiments was employed to investigate the effects of (i) exposure time (1, 2, 3 and 4 h), (ii) treatment temperature (20, 30, 40, 50 and 60 °C), (iii) initial bacterial concentration (103, 104, 105 and 106 CFU/mL) and (iv) sunlight intensity (0, 800 and 1200 W/m2) on Escherichia coli survival for a subsequent 48-h dark control period.
However, no changes in the dark control samples were observed.
Dark control group keep identical to experimental group except illumination.
Enhanced release of AF750 was observed in samples irradiated with UV versus dark control.
The study is conducted under sterile conditions with a dark control in parallel.
For each sample a dark control sample was prepared, which was protected with aluminum foil before irradiation.
