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And of course, no one will ever forget the moments when we courageously watched "Are You Afraid of the Dark?", collected Beanie Babies, and all the while kept a watchful eye on our Tamagotchis and Furbies.
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A 5 mL subsample of each groundwater sample was stained with DAPI (1 µg mL−1) for 20 min at room temperature in the dark and collected on black polycarbonate Isopore Membrane filters (0.2 µm GTBP, Millipore, Ireland) with the Millipore 1225 Sampling Manifold (Millipore, USA) under low vacuum.
A boy band that goes round after dark collecting people's teeth.
The dark scans collected in the potential range between −0.4 and 0.8 V reveal a small background current of ∼10−7 A/cm2.
The dark particles collected by centrifugation at 5000 g for 10 min were subjected to three washes using PBS followed by treatment using Reaction Buffer (10.0 mM Tris, 1.0 mM CaCl2 and 0.5 % SDS, pH 7.8) with 10 μL of 10 mg/mL Proteinase K and incubated at 37 °C.
Cells were incubated with either 1 μg/ml HO or 5 μg/ml PI at 37 °C, 5% CO2 for 15 min in the dark and collected by centrifugation.
Irradiation was performed using a ring of six LEDs affixed to the crystal cooling apparatus within 1 cm of the crystal.[ 20] Suitable single crystals were mounted on the diffractometer and cooled to 100 K. Ground-state structures ("dark") were collected with no external light.
Here, you're playing Switch, a firefly who rather than bimble around in the dark, can collect cherries, loop the loop and fire a slingshot.
No more CSA members dribbling in after dark to collect bok choy.
The other one, Dark Days, collects 14 tracks he produced as a teenager between 1992 and 1997 when he'd clearly been listening to both volumes of Aphex Twin's Selected Ambient Works on heavy rotation.
The DOC's investigation did not acknowledge the air quality of the prison or inmates' accounts of dark matter collecting in vents and on the windowsills seemingly from the dry-coal-ash dump.
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