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i-l Sections of TH4n × Nip2n developing seeds at 2 DAP i, 3 DAP j, 4 DAP k, and 5 DAP l.
f-i GUS activity in transgenic plant carrying pLOC_Os07g44430 GUS vector, using samples from whole seedling (f), flower (g), germinating seed (h), and developing seed at 3, 6, 10, 15, and 20 DAP (i).
i-l Serial sections of TH4n × Nip2n developing seeds focusing on pericarp at 2 DAP i, 3 DAP j, 4 DAP k, and 5 DAP l. m-p Serial sections of TH4n × TH4n developing seeds focusing on pericarp at 2 DAP m, 3 DAP n, 4 DAP o, and 5 DAP p. In summary, seed development between the two balanced crosses is similar, following the patterns as previously described (Brown et al. 1996; Itoh et al. 2005).
The chamber slides were mounted with "VECTASHIELD Mounting Medium with DAPI" (Vector Laboratories, Burlingame CA).
(B) HAI-178-FMNPs bound to the membrane of MGC803 cells, blue nucleus (DAPI staining) (×10).
Double iNOS/NeuN labeling (NeuN for neuronal cell bodies) and iNOS/DAPI labeling (DAPI for nuclei) revealed a clear cytosolic iNOS activity in neurons in infected rats.
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Our group recently described the protective effect of DMSO against SSB and DSB formation by 99mTc-HYNIC-DAPI.
The SeNPs@Am-induced cell apoptosis was further determined by a TUNEL-DAPI assay [31].
Stained slices were mounted to Fischer Scientific Superfrost standard microscope slides using Fluoromount-DAPI.
For determination of cell cycle distribution, AML cell lines were stained with NIM-DAPI reagent (Beckman Coulter, Fullerton, CA).
The SKY-DAPI images were captured using a Nikon microscope equipped with a Spectral cube and Interferometer module.
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