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SNVs were predicted damaging using the SIFT, Poly-Phen or the Mutation Taster software within the SVS7 pipeline.
This mutation was absent from public SNP databases (dbSNP, 1000 Genome variant catalogue), conserved and was predicted to be damaging using SIFT and PolyPhen-2 [ 30, 31].
The mutated amino acid is highly conserved across species down to invertebrates and is predicted to be damaging using PolyPhen-2 and SIFT (Fig. 2).
The p.R10T variant is predicted to be tolerated by SIFT and benign using Polyphen, p.V127W is predicted to be not tolerated by SIFT and possibly damaging by PolyPhen and p.L270F is predicted to be not tolerated by SIFT and possibly damaging using PolyPhen.
SNPs that showed a trend for association (7 SNPs with p ≤ 0.01) with either maximum BMI in adulthood or maximum BMI z score in childhood, or were predicted to be damaging using the Ingenuity Variant Analysis (rs11872992) were further genotyped in all remaining subjects (n = 3,852, mixed-heritage American Indians, Supplementary Table 1).
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Here, a method of enhancing modal frequency sensitivity to damage using feedback control is introduced.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com