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To determine the DNA damaging properties of plumbagin, HCT15 cells were incubated with 15 µM and 30 µM plumbagin for 24 h, whereas, HT29 cells were incubated with 50 µM and 75 µM plumbagin for 24 h.
One type of cellular stress is ionizing radiation due to its DNA damaging properties.
It is known that both compounds possess a broad spectrum of cell damaging properties besides the DNA damage [ 13, 14].
At concentrations up to 50 100 μM MELK-T1 did not show direct DNA damaging properties in these in vitro assays.
Despite extensive knowledge of the DNA damaging properties of Mn, little is known about the pathways involved in the response and repair of Mn-induced DNA damage.
The primary ROS generated in mitochondria can diffuse from these organelles and reach the nuclear DNA inducing its damage, or can generate secondary radicals with DNA damaging properties [ 21, 22].
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