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Spermatogenesis is threatened by DNA alkylating agents, one major category of DNA damaging agents.
Among 242 DNA damaging agents, 202 showed associations with at least one DNAR genomic signature.
These patients' cells were susceptible to DNA damaging agents.
Critically, cells derived from patients with microcephaly and seizures are sensitive to various DNA damaging agents.
Upon exposure to DNA damaging agents, Saccharomyces cerevisiae respond by activating a massive transcriptional program that reflects the fact that "DNA damaging" agents also damage other cellular macromolecules.
For instance, reduction of BRCA1 expression results in increased sensitivity to the DNA damaging agents [48], whereas overexpression of BRCA1 increases resistance to the DNA damaging agents [49].
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DNA-damaging agents leading to PARP activation were previously shown to increase FK866 activity [6], [7].
Lee, W. et al. Genome-wide requirements for resistance to functionally distinct DNA-damaging agents.
Conversely, polyploid cells may be resistant to DNA-damaging agents.
Briefly, cells were plated in 6-well plates and treated with indicated DNA-damaging agents.
As a result, TAX limits cell cycle arrest induced by exogenous DNA-damaging agents.
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