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Oxidative damage was analysed in tumor tissue using CellRox Green (Invitrogen) according to the manufacturers' recommendations.
The DNA damage was analysed by single cell gel electrophoresis (comet assay).
DNA damage was analysed by histone γH2AX and Comet assays.
DNA damage was analysed by the comet assay according to the alkali-alkali (A/A) method as described by Menke et al. [ 21].
Microvascular damage was analysed and quantified using the criteria established by Cutolo et al. Essentially, dcSSc patients were graded as having an early (E), active (A) or late (L) pattern of capillary damage [ 35].
BMPCs and PBMCs were treated ex vivo with 100 μg ml−1 melphalan for 5 min at 37 °C in RPMI-1640 supplemented with 10% FBS, penicillin (100 U ml−1), streptomycin (100 μg ml−1) and 2 mmol l−1 L-glutamine, followed by incubation in drug-free medium for 1 h and DNA damage was analysed thereafter.
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Qualitative analysis of surface and subsurface damage is analysed using SEM photographs.
The near field surrounding the damage is analysed with the 3D FE method.
The design philosophy regarding fatigue damage evaluation is illustrated with the offset of wind turbine rotor blades, and the tension fatigue damage is analysed for this particular family of textile composites.
Then, the influence of a number of factors, including mechanical and in situ stress anisotropy, as well as excavation geometry, on the simulated damage is analysed for three different geomechanical scenarios.
Its deformation and damage behaviour was analysed by means of experimental assessment of damage evolution based on single-fibre failure and finite-element simulations.
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