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The important role of XPA is thus damage verification to ensure that repair occurs only at the point of lesion [ 9].
For final damage verification, the base needs to be flipped out of the DNA helix and accommodated in the active site cavity of the glycosylase.
This site is important for (i) enabling and controlling 5'-3' translocation and force generation by XPD, (ii) for positioning the ss-dsDNA junction near the strand separating wedge, and (iii) for damage verification by XPD (Mathieu et al. Curr. Biol. 2013).
NER is initiated by two damage-recognition pathways, transcription-coupled (TC -NER and global genome (GG)-NER, which remove damage in roughly four successive sTC -NER) lesion detection, 2) locandunwindinglobaldamagenomeification, 3) excision of the DNA surroundinGG -NEResion, and finally 4) DNA synthesis and liGG -NERto fill the resulting gap [ 20].
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The paper is a sequel to Hassan and Batra's paper [Composites B, 2007] wherein details of the damage model, verification of the code, and the validation of the mathematical model are given.
The rate-limiting steps in the NER pathways are DNA damage identification and verification.
Composites B, doi 10.1016/j.compositesb.2007.02.001] wherein details of the damage evolution equations, verification of the code, and the validation of the mathematical model are given.
In the numerical verification, the damage quantification performance of the proposed method is compared with the performance of other existing methods.
Histological verification of damage has been touted as the "gold standard" as it allows visualization of morphological changes that can be appreciated qualitatively [ 50].
The experiment consisting of five different sizes of damage is carried for method verification and the identified results show the efficiency of the proposed method.
These projects aim to develop an integrated durability solution for engineering components at elevated temperature, and involve material characterisation, software development of numerical damage models, software validation and verification.
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