This suggests that the nucleoplasmic fraction of SLX4 is recruited to DNA damage tracks, where SIMs enable SLX4 to bind to SUMOylated proteins at damaged DNA.
And they must run them slower because extreme cold can freeze switches and damage tracks.
Both FUS mutants showed significantly reduced recruitment at the damage track (Fig. 7a, b, Supplementary Movie 1, 2 and 3).
FUS was previously shown to be rapidly recruited to UVA irradiation-induced DNA damage tracks in a PARP-dependent fashion17,18,19.
Recruitment of the GFP fusion proteins to MIR-induced DNA damage tracks was monitored in live cells.
To further dissect the role of FUS in this early oxidative DNA damage response, we examined the effect of FUS depletion on XRCC1 recruitment to MIR-induced DNA damage tracks.
Independent studies demonstrated that FUS is recruited to DNA damage tracks by microirradiation (MIR) at wavelengths of 405 nm or 351 nm (UVA), respectively, in a PARP1-dependent manner presumably via interaction with PAR groups17,18,19.
"Beginning in Laura's home studio, a natural sway towards Electronica took their combination of bedroom Electro and quirky lyrics from minds to myspace and soon Disco Damage tracks were being broadcast on airwaves reaching America, Australia, Japan and all around".
DNA damage tracks were generated with a Mira modelocked titanium sapphire laser.
As previously described, NBS1-mCherry was rapidly recruited to laser-induced DNA damage tracks 22.
