Sentence examples for damage to the template from inspiring English sources
The most likely reason is the damage to the template that results from exposure to ethidium bromide and UV light.
The former mechanism is unlikely, because we have directly checked the effect of psammaplin A on DNA and didn't find any detectable damages to the template (data not shown).
Furthermore, Malins et al. have described progressive alterations in DNA structure between normal, BPH and cancerous prostate tissues due to oxidative damage to the DNA template be hydroxyl free radical [ 36- 38].
As the theoretically expected number of mismatches based on the sequencing error rate is 1.6%, other sources of error (such as damage to the original DNA template, sequencing errors or mtDNA heteroplasmy) may be affecting the results.
DNA replication is sensitive to damage in the template.
Cloning is generally performed in the case of aDNA studies to detect mosaic sequences as a result of jumping PCR between sequences, to identify amplification errors due to post-mortem DNA damage in the template and to detect PCR-slippage in the case of microsatellites [ 20, 54].
Transcription elongation complexes tend to pause or stall when they encounter a protein road-block or DNA damage on the template strand, reducing the transcription of downstream sequences.
In this study the yield is analyzed as a function of several processes: (1) thermal damage of the template and polymerase occurring during the denaturing step, (2) competition existing between primers and templates to either anneal or form dsDNA, (3) polymerase binding to annealed products (primer/ssDNA) to form ternary complexes and (4) extension of ternary complexes.
