DOI: http://dx.doi.org/10.7554/eLife.09623.030 In response to the concern regarding the inhibition of DNA damage and specificity of Erk inhibition, we stimulated BMDCs with nCB (PBS was used as control) in the presence or absence of Ku55933 (ATM inhibitor), Nu7026 (DNA-Pkc inhibitor) or different concentration of U0126 (MEK inhibitor) for 24 hours.
However, we found that though sensitivity of IPL and STG damage were comparable, specificity of IPL damage as a predictor of spatial neglect was significantly higher than of STG damage.
Different DNA glycosylases, including UDG, remove different kinds of damage, and the specificity of the repair pathway is determined by the type of glycosylase involved.
Although urinary KIM-1 is highly sensitive to (proximal) tubulointerstitial damage, it lacks specificity in the presence of other (chronic) comorbid organ dysfunction.
For S-100β, the manufacturer (Roche Diagnostics GmbH, Mannheim, Germany) proposes a cutoff of 0.105 μg/L on a detection range of 0.005 to 39 μg/L for patients with possible cerebral damage (sensitivity 99%, specificity 33%).
H-FABP has shown to be a far more sensitive biomarker when compared to TnT within the first 6 hours after surviving acute heart damage; however, its specificity is reduced after 6 hours [ 50– 50].
Figure 2a shows that transfection with the three Sik3 siRNAs increased the mitotic index in NIH3T3 fibroblasts irrespective of the presence or absence of Adriamycin-mediated DNA damage, supporting the specificity of the mitotic effects for Sik3.
p53 has been shown to be activated by both CHK2 and CHK1 in response to DNA damage; however, the specificity of CHK1 versus CHK2 activation can be distinguished because CHK2-dependent activation of p53 results in the phosphorylation of Ser20 of p53, whereas CHK1 activation results in the phosphorylation of Ser15 of p53.
This paper presents a composite damage behavior law whose specificity is to take into account the variability of some material parameters.
Further studies must be conducted to determine the specificity of damage and biomarkers for individual disease states.
