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Culture was maintained for 3 4 weeks in the absence of drug selection with daily medium changes.
Forty-eight hours after transfection, cells were treated with 2μM 6-thioguanine (6-TG; Sigma) for 5 days, with daily medium and drug replacement.
After 3 5 more days of daily medium changes with NuFF-1 MITC-conditioned Pluriton medium, visible colonies were counted on AP-stained and unstained wells.
Twenty-four hours after seeding, cells were treated with 100 nM topotecan for 5 days with daily medium and drug replacement.
To standardize the kairomone concentration for all kairomone treatments, medium was enriched with Notonecta kairomones and pooled prior to the daily medium change.
Forty-eight hours after transfection, cells were treated with 2 μM 6-TG for 5 days, with daily medium and drug replacement.
Similar(31)
In further analyses, we additionally adjusted for daily medium-sized servings of vegetables (< 1.5, 1.5 2.9, ≥ 3), and daily medium-sized servings of grains (quartiles).
As shown in Figure 4, a recombinant mAb-expressing CHO cell line was treated with the inhibitor using a plate-based production format, which includes complete daily medium-exchange.
In addition, we used c value of 2.3 per day, which is estimated from daily harvesting of viruses (i.e., the amount of p24 have to be reduced by around 90% per day by the daily medium-replacement procedure).
A daily full medium change was performed with warm STEMdiffTM Neural Induction Medium.
βFGF was added daily and medium changed every two days.
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