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The analytical HPLC was performed using an Agilent 1200 Series apparatus equipped with a quaternary pump, an autosampler, a DAD detector (detection wavelength of 270 nm, reference wavelength of 360 nm, slit length of 4 nm), a vacuum degasser, a column oven and Agilent Chemstation software.
The HPLC system used for formulation potency check was an Agilent HP 1100 HPLC equipped with diode array (DAD) and variable wavelength UV (VWD) detectors and a quarternary solvent delivery system (Palo Alto, CA).
> Examples of the chromatograms of 18 standards obtained using UV-DAD detection with wavelength programming and fluorimetric detection as well as of a chromatogram of the PAH-rich fraction isolated from a sample of road asphalt 50/70 according to the procedure discussed in this paper obtained using UV-DAD and fluorimetric detection are shown in Fig. 4.
The detection wavelength of DAD was 254 nm.
The HPLC system used for formulation potency check was an Agilent HP 1100 HPLC equipped with a diode array (DAD) and a variable wavelength UV (VWD) detectors, and a quaternary solvent delivery system (Palo Alto, CA).
The mobile phase flow rate kept constant at 0.2 ml/min while monitoring with DAD at 280 nm wavelength.
Metabolites analyzed by TOF LC-MS were quantified with a diode array detector (DAD) over an extracted wavelength range of 275 to 280 nm with the Applied Biosystems Analyst QS 1.1 software package (Life Technologies, Carlsbad, CA).
Detection wavelength for the DAD was set for 288 nm with the spectrum detection range 190-700 nm.
The detection wavelength was selected by DAD according to max UV absorption of each reference.
A DAD detector was used at a wavelength of 220 nm.
A Beckman 125 HPLC instrument (Beckman, USA) equipped with a Hamilton auto sampler and a 168 photodiode array detector (DAD) was used and the detection wavelength was set at 254 nm.
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