In contrast to the plots discussed before, we now have drawn the plot with respect to the LCC's I/ Qplane, i.e. the CCU1 DAC values.
The validation threshold (dashed line in figure) was set to 12 DAC values higher than the previous one to discard signals generated by dark noise but to accept a high fraction of the signals generated by the LYSO background.
For 23-12, the "reproductive process" genes were detected significant decrease at 25 DAC (p value was 0.0335), which was almost no change for 25-5.
In the high-order DAC algorithm, this value remains invariant during each iteration since (11).
Adventitious root formation is a complex process regulated by many factors, the "signaling process" genes were significant increase at 25 DAC for 25-5 (p value was 0.0241), but almost no change for 23-12, whindicatedated signaling process involved in regulation of adventitious root meristem formation (25DAC).
For 25-5, the "catalytic activity" and "binding" genes were detected increased at 25 DAC than 14 DAC (p value were 0.0307 and 0.0484, Additional file 2), which was almost no change for 23-12.
We assume that the TX filter after the DAC does not have a higher Q value, which is reasonable for broadband DACs, not requiring steep filtering between DAC and PA.
The greater potency of DAC versus AZA, based on calculated EC50 values, does not take into account the plateau effect on cell viability observed with DAC, in which increasing drug concentrations above 1 µM did not lead to a further reduction in cell viability below 40% after 3 days.
The detailed information of the number of detected genes between 14 DAC and 25 DAC and the p values of each GO category were shown in Additional file 2. The profiles of proteins extracted from cutting tissue on 14 DAC, 25 DAC, and 35 DAC were investigated using a proteomics approach.
