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Goat serum and the DAB substrate system were purchased from Santa Cruz Biotechnology.
Immunoreactive proteins were visualized by a DAB substrate solution (Kirkegaard & Perry Laboratories, Inc., Gaithersburg, MD).
The staining's were visualized by using an ImmPact™ DAB substrate kit (Vector).
DAB substrate Kit was from Zymed Laboratory Inc. (Carlsbad, CA).
The staining was developed using DAB substrate system.
The peroxidase activity was developed using a DAB substrate kit (ImmunoPure; PIERCE Biotechnology, Rockford, IL, USA).
Sections were visualised with diaminobenzidine (DAB substrate kit, Vector Labs Inc).
For immunohistochemistry, slides were incubated with DAB substrate kit for peroxidase (Zymmed laboratories).
Vectastain Elite and ImmPact DAB substrate kits (both from Vector labs) following manufacturer's instructions.
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Lyve-1 was visualized with the Vectastain kit followed by DAB substrate-chromogen (Vector).
Immunostaining was developed using the peroxidase-labelled polymer and 3,3′-diaminobenzidine (DAB) substrate-chromogen solution.
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