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Considering the reproducibility, we recommend to use the commercial DAB Kit (e.g., CWBIO here) for DAB staining.
Pseudocolor images use red as high-density DAB staining, orange as moderate, and yellow as low-density DAB staining.
Finally, slides were visualized by 3,3-diaminobenzidine (DAB) staining.
These images were analysed with ImageJ plugin ImmunoRatio to calculate the percentage of DAB staining.
The H2O2 production was detected by the 3,3′-diaminobenzidine tetrahydrochloride (DAB) staining.
Histochemical detection of H2O2 was done by 3,3′-diaminobenzidine (DAB) staining as described by Thordal-Christensen et al. (1997).
(B) Localization of H2O2 in the leaves of tomato by DAB staining method at 24 h of FA treatment.
These data are consistent with the H2O2 DAB staining.
The reaction product was developed with DAKO DAB staining kit, dehydrated and mounted with DPX.
To investigate the oxidative state of leaves of the Rils further, the levels of ROS were examined by DAB staining.
One week later, neurons were fixed in 4% paraformaldehyde and immunostained for GFP followed by DAB staining.
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