Da, da, da, da, da, da: the first photo from the "Gilmore Girls" revival is HERE. .
The serum level of HBV DNA was assayed by a fluorescent quantitative detection kit (Da An Gene, Guangzhou, China).
A commercial licensed kit (Da An Gene Co. Ltd, lot no: Cox A16 YZB-0354-2009, EV-A71 YZB-0356-2009) was recommended by China CDC for detection of EV-A71 and CV-A16.
The Toxoplasma-specific IgG antibodies in 3181 serum samples were tested by Indirect Enzyme Linked Immunosorbant Assay (ELISA) [ 17] and the results were further verified by direct agglutination test with the Toxo-Screen DA (BioMerieux, France) kit.
Mice sera were collected, and an HBV fluorescence quantitative detection kit (Da-An, Guangzhou, China) was used according to the instructions.
The real-time fluorescent quantification PCR (FQ-PCR) was used to measure the copies of HBV-DNA according to the manufacturer's recommendations of HBV-DNA test kit (Da-An Gene Co., Guangzhou, China).
The load of HBV viral was determined using a HBV Fluorescent Quantitative PCR Detection kit (Da-An Gene Co., Ltd., China) according to the manufacturer's protocol.
Serum HBV-DNA was measured using a commercially available real-time fluorescent quantification PCR (FQ-PCR) diagnostic kit (Da-An Gene Co., Guangzhou, China) according to the manufacturer's instruction.
The instrument was externally calibrated prior to analysis using the Bruker protein calibrant kit (5000 17000 Da), and the sample spectra internally recalibrated according to the accurate molecular weight of three antennal proteins (8015, 11941 and 13936 Da) measured in the FTMS analyses.
