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Fructose-drinking rats received D-fructose (100 g/l).
Fructose dehydrogenase (FDH) catalyzes oxidation of d-fructose into 2-keto-d-fructose and is one of the enzymes allowing a direct electron transfer (DET -type bioelectrocatalysis.
The kon values with various sugars follow the order of d-fructose > d-tagatose > d-mannose > d-glucose.
The main common reaction path is from C00267 (α- d-glucose) to C00018 (pyridoxal phosphate) through C00794 (Sorbitol), C00095 (d-fructose), C05345 (β- d-fructose 6-phosphate) and C00118 (glyceraldehyde 3-phosphate).
d-Fructose dehydrogenase (FDH) from Gluconobacter japonicus NBRC3260 catalyzes the two-electron oxidation of d-fructose to 5-keto-d-fructose, and it is widely used in biofuel cells and biosensors.
The hyperuricemic effect of D-tagatose, a stereoisomer of D-fructose, may be greater than that of D-fructose, as the subsequent degradation of D-tagatose-1-phosphate is slower than the degradation of D-fructose-1-phosphate.
Moreover D-fructose in a mixture of D-fructose and D-glucose could be "knocked-out" by adding phenylboronic acid since it interacts strongly (selectively binds) with D-fructose [19].
The thermogenic and lactacidemic responses to D-tagatose were blunted compared with D-fructose.
D-fructose was considered as an internal standard in the present study.
Figure 3 Molecular sensor forms amorphous conjugates in the presence of D-fructose.
The pyrene-boronic acid derivative produced amorphous 1 1 conjugates in the presence of D-fructose.
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