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Analysis of mOEC cytoplasmic volume after treatment with RAD288 and RAD289.
(b) Cytoplasmic volume of mOECs after treatment with RAD288 at 3.13 and 6.25 µM.
(c) Cytoplasmic volume of mOECs after treatment with RAD289 at 3.13 and 6.25 µM.
RAD288 and RAD289 change OEC morphology by increasing the cytoplasmic volume.
RAD289 at 6.25 µM has strong effects both on cell proliferation and cytoplasmic volume.
A 2.2% increase in cytoplasmic volume on mOECs treated with 6.25 µM of RAD288 was observed (p < 0.05) (Fig. 5b).
Several factors can be responsible for changes in nucleus morphology, including DNA content, cytoplasmic volume, nucleocytoplasmic transport and nuclear structural components25.
Vasopressin levels, cytoplasmic volume and c-Fos expression returned to control levels by 18 weeks after landing.
Our results also showed that mOEC cytoplasmic volume increased by 11% (p < 0.05) and 15% (p < 0.01) after treatments with 3.13 and 6.25 µM of RAD289, respectively (Fig. 5c).
"For each cell, the corrected fluorescence intensity and the known cell geometry then enables estimation of the average ribosome number density (i.e. the average concentration over the entire cytoplasmic volume) and the peak ribosome number density in the ribosome-rich regions..
The hexanic and aqueous soluble fraction in the methanol fractions promoted changes in the intertubular compartment because they increased the nuclear volume, cytoplasmic volume and individual volume of Leydig cells in male Wistar rats [57].
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