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Positive cells were ring-isolated using cloning cylinders, expanded in culture for 4 7 days and re-screened until the majority of cells were found to be positive.
Individual cell colonies were picked using cloning cylinders, expanded to 75 cm tissue culture flasks and screened for FGF1 expression by qRT PCR analysis, as previously described.
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The engine Cadillac put in the Elmiraj is a conceptual product as well, a projected version of the company's twin-turbo 6-cylinder, expanded into V-8 form and rated at 500 horsepower.
Furthermore, as the cylinder expands during lithiation it can develop a compressive axial stress large enough to induce buckling, which in turn may lead to mechanical failure.
As in the apical meristem, the number of dividing cells in the cambium remains constant, except that more cells occasionally are added by divisions in the radial plane so that the girth of the cambial cylinder expands in pace with the growth of the xylem within.
Three weeks after the drug selection, colonies were harvested with cloning cylinders and expanded to cell lines.
Blasticidin resistant colonies were picked using cloning cylinders (Sigma), expanded, frozen and maintained at − 150 °C until further use.
Compressed air enters the cylinder, expands, and forces the piston to move.
Following selection with G418, distinct clones were picked using cloning cylinders and sequentially expanded in 12-well plates, and then T25 and T75 flasks prior to performing functional analyses and generation of frozen stocks.
This means that as the space in one cylinder is expanding, the space in the other is contracting.
The cylinder pressure force is expanded in a Fourier series for evaluating the bearing characteristics, establishing the balancing and predicting the response of the entire compressor.
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