Sentence examples for cycling steps from inspiring English sources

PCR was performed on a Bio-Red thermocycler (DNA Engine) and included the following cycling steps: 3 min at 95°C, 20 25 cycles of 40 sec at 94°C, 30 sec at 60°C, 45 sec at 72°C; and a final 10 min extension.

A schematic representation of the cycling steps for real-time LCR is shown in Figure 3.

At the end of PCR cycling steps, data for each sample was displayed as a melting curve.

The initial selection of temperatures for real-time LCR cycling steps was based on melting simulations for each candidate LCR oligonucleotide and LCR product, with their respective complements.

The light cycler was programmed to the following conditions: an initial PCR activation step of 10 min at 95°C, followed by cycling steps; denaturation for 15 sec at 95°C, annealing for 30 sec at 60°C, and elongation for 60 sec at 72°C; these steps were repeated for 40 cycles.

The temperature transition rate for all cycling steps was 20°C/s.

For example, a combination of rowing, kayaking, cycling, stepping on a stepping machine or using an elliptical trainer.

Data for each cycle was acquired after the final cycling step.

Consequently, the estimated melting temperature was 66°C for probes and 56°C for primers, and an additional cycling step of 50°C was added to the real-time PCR.

A cold centrifugation was performed on the dialyzed protein, and a final temperature cycling step was performed to ensure removal of guanidine.

The RT-PCR was performed under the following steps: initial denaturing step at 94 °C for 5 min, 35 thermal cycling step consisted of 30 s at 94 °C, 30 s at 56 °C and 30 s at 72 °C, and then final extension step at 72 °C for 5 min for termination.