Sentence examples for cycling step from inspiring English sources

Data for each cycle was acquired after the final cycling step.

Consequently, the estimated melting temperature was 66°C for probes and 56°C for primers, and an additional cycling step of 50°C was added to the real-time PCR.

A cold centrifugation was performed on the dialyzed protein, and a final temperature cycling step was performed to ensure removal of guanidine.

Amplification and detection were performed on the ABI 9800 Fast Thermal Cycler Applied Biosystemm) using for both primer sets, the same cycling conditions with an initial cycling step at 94°C for 10 minutes followed by 94°C, 55°C and 72°C for 30 seconds each ending at 4°C.

The RT-PCR was performed under the following steps: initial denaturing step at 94 °C for 5 min, 35 thermal cycling step consisted of 30 s at 94 °C, 30 s at 56 °C and 30 s at 72 °C, and then final extension step at 72 °C for 5 min for termination.

We then identified candidate mediators and their most plausible conceptual ordering by systematically exploring the associations between the environmental perception measures (factor scores for the physical environmental items, and the mean change score for the visibility items), proximity to and use of Connect2, and change in time spent walking and cycling (step 2).

For example, a combination of rowing, kayaking, cycling, stepping on a stepping machine or using an elliptical trainer.

PCR was performed on a Bio-Red thermocycler (DNA Engine) and included the following cycling steps: 3 min at 95°C, 20 25 cycles of 40 sec at 94°C, 30 sec at 60°C, 45 sec at 72°C; and a final 10 min extension.

A schematic representation of the cycling steps for real-time LCR is shown in Figure 3.

At the end of PCR cycling steps, data for each sample was displayed as a melting curve.

The initial selection of temperatures for real-time LCR cycling steps was based on melting simulations for each candidate LCR oligonucleotide and LCR product, with their respective complements.