A new design of a mechanical field-cycling setup, operating in the wide temperature range up to 1200 K has been implemented.
The reaction setup, cycling program, standard curve method and primer pairs for Angptl4, Dnajb1 and Tfrc have been described before [27].
Information distributed to laboratories asking for assistance with CHIKV RT-PCR included reaction chemistry setup, cycling profile, and primer and probe sequences.
Amplification kinetics is determined by a combination of reaction setup and cycling regime.
An aliquot of 2 μl per serum DNA isolate served as template for the reaction and the 2-step -PCRR reaction setup and cycling was performed as published previously [ 2-step -PCR
Thus, amplification efficiency must be determined for every new amplicon, and re-determined if changes are made to reaction setup and/or cycling regime.
The primer sequences, the reaction setup and the cycling conditions were the same as described above.
We can directly setup the next cycling programs with lower annealing temperature in the phase of amplification of fused DNA fragments, but it is no need to make a new PCR reaction solution.
An application is demonstrated, combining the DNP setup with a commercial fast field cycling NMR relaxometer.
THE SETUP -- There will be 18 cycling events: 12 on the track, four on the road and two for mountain bikes.
