Sentence examples for cycling procedure from inspiring English sources

The cycling procedure was 35 cycles of denaturation at 94°C for 45 s, annealing at 56°C for 45 s and extension at 68°C for 8 min with 2 min of pre-denaturation and 10 min of final extension.

The kinetics of these processes show a considerable increase of the associated time constant after the pseudoelastic cycling procedure.

The cycling procedure was 94°C for 5 min, then 35 amplification cycles (94°C for 45 s, 56°C for 45 s, 72°C for 2 min), followed by a final extension step at 72°C for 10 min. The resulting amplicon was gel-purified using QIAQuick gel extraction kit (Qiagen).

Copolymersof aniline and N- 3-sulfopropyl)aN- 3-sulfopropylre electrosyN- 3-sulfopropylotentianilineiNSPArocedure.

The PCR cycling procedure was as follows.

A melting curve was performed at the end of the cycling procedure to ensure single product amplification.

Since cogwheel phase cycling procedures can be designed for a range of scan numbers, and can be combined with pulsed field gradients, the total experiment time can be adjusted closely to the required signal-to-noise ratio with minimal overhead.

Specific primers of the marker DNA combined with the degenerated primers allow amplification of DNA fragments adjacent to the marker insertion by using two rounds of either short or long cycling procedures.

The first stage defines the experimental campaign on a mixing at improved conductivity, via the SolTeCa experimental system, with review of the storage elements geometry, location of thermocouples and cycling procedures.

A surface layer formation on positive Li1 + xMn2O4 − δ thin film model electrodes as a result of electrochemical cycling procedures has been detected and characterized by scanning electron microscopy and X-ray photoemission spectroscopy.

The sequences of primers and thermal cycling procedures are shown in Additional file 1: Table S1.