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The filter was returned to the manifold and individual wells were incubated for 1 hr with 100 µL of two-fold serial dilutions of affinity-purified rabbit antibodies (AbSV linear, Ab4L, AbHV2 linear; range 8.2 720 ng) or mouse antisera (Absv cyclic, AbHV2BD cyclic; range 1∶20 1∶320).
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The gauge factors for an intermittent cyclic loading (range: 2.85 3.37) are larger than those for a continuous cyclic loading (range: 1.89 2.16).
It is seen that the reliability depends on the maximum stress level as well as the cyclic stress range.
The influence of friction between punch and sheet on the magnitude of the cyclic stress range and mean stress that act locally at the tool surface was investigated.
Stress ratio effect is intrinsically considered in the proposed fatigue model since the stress state is directly used instead of using the cyclic stress range.
The effects of initial condition as produced by cold working or annealing, cyclic strain range, frequency and degree of monotonic prestrain on subsequent fatigue behavior were investigated.
The relationship between the number of cycles to failure of pure fatigue tests and the cyclic strain range is established for pure fatigue tests.
The lifetime as a function of thermal cyclic strain range for the narrow Au lines follows the conventional Coffin Manson relationship when Δε ⩽ 0.47%, while the number of cycles to failure extends to an engineering-defined high-cycle fatigue region.
The number of cycles to failure increased as the cyclic load range decreased in a manner similar to that found in many engineering materials: fatigue occurred at applied loads as small as 40% of the ultimate strength.
The effects of the fiber volume fraction, fatigue peak stress, matrix cracking space, fiber/matrix interface debonded energy and cyclic temperature range on the thermomechanical fatigue hysteresis loops subjected to out-of-phase cyclic loading have been analyzed.
The development of scattered damage in a composite over the cyclic load range and during loading and unloading was studied by acoustic emission in Part 1 of this work.
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