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P23H-3 (P23H line 3) and control Sprague Dawley (SD) rats were raised in cyclic light (12 h light, 12 h dark), with the light phase set at either 5 lx ('scotopic-reared') or 40 60 lx ('mesopic-reared').
(6 Fluid elements move between the relatively low and highly illuminated zones intermittently and undergo a cyclic light effect (Li and Yang 2013).
In that study the mice were raised under cyclic light, making a direct comparison to our study difficult.
Mice were maintained in the breeding colony under cyclic light (14-hour light/10-hour dark) conditions; cage illumination was approximately 7 foot-candles during the light cycle.
Rats were kept in cyclic light (12 hours ON/OFF: 200 lux/dark) after birth and fed laboratory chow ad libitum with free access to water.
A recent study reported that albino rats exposed to intense cyclic light developed photoreceptor damage and CNV in a relatively short time frame [20].
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Mice were maintained up to 6 weeks of age in cyclic lighting or in total darkness.
Previous study suggests that the juvenile horseshoe crabs are also found to be nocturnally active under natural cyclic lighting and maintained rhythmic activity in constant darkness (Borst and Barlow 2002).
This discrepancy may be due to differences in experimental protocols (cyclic light-reared for P347S vs. dark-reared for Q344ter) or the nature of the rhodopsin mutation itself.
Conversely, light deprivation often exhibits a sparing effect compared to standard cyclic lighting conditions [1], [2], [3], [4], [5], [6], [7], [8].
Wild-type (WT) C57Bl/6 mice, shown for comparison in this study, and additional L52H transgenic mice were also reared under cyclic lighting.
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