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Pre-loaded and virgin (no pre-loading) ECC coupon specimens were also exposed to freezing and thawing cycles in the presence of de-icing salts for 25 and 50 cycles to determine their residual tensile behavior.
In this study, TMW underwent one year of natural weathering conditions and on the other hand, eighty accelerated aging cycles, to determine potential changes of relevant properties of TMW in service.
1-5 µl of samples were subjected to PCR amplification with 40 cycles to determine the genotypes.
To the macrophages in the monolayer, 200 µL of RPMI was added and the cells were disrupted by three freeze-thaw cycles to determine the cell-associated cytokine contents.
We used 100 ng of cDNA for PCR amplification, and amplified all of the sequences with multiple cycle numbers (25 27 cycles) to determine the appropriate conditions for distinguishing semi-quantitative differences in expression levels.
The Markov model included 23 cycles to determine costs and effects over lifetime.
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This method will be used to analyze the cycle, to determine which components need to be optimized.
The primary emphasis is placed on analyzing the ideal cycle to determine the upper limit of the engine.
Next, we cultured OPCs for 24 h in the presence of EdU (5-ethynyl-2′-deoxyuridine), a marker of cells in S-phase of the cell cycle, to determine whether the proliferative effect of growth factors was modified by cholesterol.
This time can be compared with the period of the lean A/F operation during the aging cycle to determine the location and duration of high-temperature lean exposure.
Tracking your cycle to determine a fertile window has long been used to either become pregnant or avoid conceiving.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com