Sentence examples for cycles target from inspiring English sources

Daughter nuclide elution follows in pre-determined cycles Target material irradiation by charged particle beams.

RT-qPCR amplification conditions included an enzyme activation step of 95°C (10 min) followed by 40 cycles (reference genes) or 50 cycles (target genes) of 95 C (15 sec) and a primer specific combined annealing/extension temperature (1 min).

PCR conditions were one 5-minute cycle at 95°C, 25 (β-actin) or 30 cycles (target mRNAs) each comprising a 30-second step at 95°C, followed by a 30-second step at primer-specific Tm and a 45-second step at 72°C, and one last cycle of 7 minutes at 72°C.

To achieve simple and effective multiplexing of targets in one qPCR reaction, we have combined low-cycle-number (<15 cycles), target-specific multiplex amplification with single-molecule amplicon detection using atomic force microscopy (AFM).

A New Cell-cycle target in cancer inhibiting cyclin D-dependent kinases 4 and 6.

The loss in compressive strength increases as the cycle target temperature increases from 200 °C to 800 °C.

Several operation scenarios are considered, including core water flow variation throughout the cycle, target different axial power distributions and Haling conditions.

We performed complementary DNA synthesis and labeling and array hybridization by following the Affymetrix user's manual with a one-cycle target labeling and control reagent kit (Affymetrix, Santa Clara, CA, USA) with 0.4 μg of mRNA as the template material.

Biotin labeling of target RNAs was performed with one-cycle target kit (One-Cycle Target Labeling and Control Reagents, Affymetrix, P/N 900493), and hybridized according to the manufacturer's protocol.

In vitro transcription and labeling were performed using One-Cycle Target Labeling and Control Reagents (Affymetrix, Santa Clara, CA) for ESCs and ESC-derived cells, and using Tow-Cycle Target Labeling and Control Reagents (Affymetrix) for adult HSCs.

Total RNA was used for cRNA synthesis through two-cycle target labeling kit (Affymetrix) according to the manual manufacturer.