The optimal cycling conditions were 20 s at 95°C and 40 cycles each of 3 s at 95°C and 30 s at 60°C.
Thermal cycling conditions included activation at 95°C (10 min) followed by 40 cycles each of denaturation at 95°C (15 sec) and annealing/elongation (1 min) at 60°C.
Thermal cycling parameters included activation at 95°C (1 min) followed by 40 cycles each of denaturation at 95°C (15 sec) and annealing/extending at 60°C (1 min).
The cycling conditions were as follows: 5 min at 95 °C, followed by 45 cycles each of 15 s at 95 °C, 60 s at 60 °C.
Cycling conditions were 1 cycle at 94°C for 5 min; then 35 cycles each of 94°C (1 min), 50°C (1 min), and 68°C (1.5 min); followed by final extension of 7 min at 68°C.
Thermal cycling conditions were 2 min at 50 °C, 10 min at 95 °C and 40 cycles each of 95 °C for 15 s and 50 °C for 1 min.
For the Greeks, like the Babylonians before them, the year consisted of twelve "lunations," or new-moon-to-new-moon cycles, each of which lasted an average of twenty-nine and a half days.
In subsequent cycles, each of which adds two carbon atoms to the molecule via 64], successively longer β-oxoacyl-S-ACP derivatives are produced.
In the following century, the Sefer ha-temuna ("Book of the Image") appeared in Spain and advanced the notion of cosmic cycles, each of which provides an interpretation of the Torah according to a divine attribute.
A team of 56 scientists led by sedimentologist Tim Naish of the Antarctic Research Centre in Wellington, N.Z., identified 38 sedimentary cycles, each of about 40,000 years' duration, in good accordance with the same cyclicity recorded in marine-isotope records of global ice volume and mean deep-sea temperatures.
The compound load consists of elementary sub cycles, each of them representing one motion phase.
