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Cycling conditions were as follows: 10 min on 95°C, 40 cycles consisting of 94°C for 15 s and 60°C for 1 min.
Following cycling conditions were used denaturation at 95 °C for 5 min, 35 cycles consisting of denaturation at 94 °C for 15 s, annealing at 60 °C for 15 s, and extension at 72 °C for 10 min.
The Telomere thermal cycling profile proceeds as follows: 95°C for 10 minutes then 30 cycles consisting of 95°C for 15 seconds and 54°C for 2 minutes.
The 36B4 thermal cycling profile proceeds as follows: 95°C for 10 minutes then 30 cycles consisting of 95°C for 15 seconds and 58°C for 1 minute and 10 seconds.
The following cycling protocol was used: 10 min at 95°C followed by 40 cycles consisting of 10 s at 95°C and 40 s at 60°C.
Cycling conditions were: initial denaturation at 95°C for 2 minutes; then 40 cycles consisting of denaturation at 95°C for 15 seconds, annealing at 60°C for 30 seconds and extension at 72°C for 1 minute.
The following cycling parameters were used: initial denaturation at 94 °C for 30 s; 7 amplification cycles consisting of denaturation at 94 °C for 25 s, annealing and extension at 72 °C for 3 min; 32 amplification cycles consisting of denaturation at 94 °C for 25 s, annealing and extension at 67 °C for 3 min; final extension at 67 °C for 7 min.
from saline using 11 consecutive cycles consisting of a pairing day and three nonpairing days.
Analyze at least thirty recent key sales cycles (consisting of an equal number of won and lost accounts) and map out all of the people who were involved.
from saline in eight consecutive cycles consisting of a pairing day (PD) and three nonpairing days (NPDs).
The template was denatured for 5 min at 94 °C, and DNA was amplified for 30 cycles consisting of 1 min at 94 °C, 1 min at 50 °C, and 1 min at 72 °C.
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