Sentence examples for cycles comprised of from inspiring English sources

The qRT-PCR thermal cycling conditions were 95°C for 30 sec followed by 50 cycles comprised of 95°C for 5 sec, a gene-specific temperature for 10 sec (Table  4), and 72°C for 10 sec.

The reaction was performed using the program consisting of a denaturing cycle at 95°C for 5 min; 20 cycles comprised of 95°C for 50 s, 60°C for 50 s, and 68°C for 6 min and a final step of 8 min at 68°C.

The PCR amplifications were performed as follows: initial denaturation of DNA at 94°C for 5 min, 35 cycles comprised of 1 min denaturation at 94°C, 1 min annealing at 55°C, 2 min elongation step at 72°C followed by a final extension step at 72°C for seven min. Amplicons were visualized by electrophoresis on 1% agarose gel stained with ethidium bromide.

Amplification conditions included an initial denaturation at 94°C for 2 min followed by 25 cycles comprised of 94°C for 30sec., 55°C for 30 sec., and 68°C for 2 min. PCR products were resolved in 1% agarose gels with ethidium bromide.

Amplification conditions included an initial denaturation at 94°C for 15 min followed by 39 cycles comprised of 94°C for 1 min., 54°C for 30 sec., and 72°C for 2 min. The reaction was completed by a final elongation step at 72°C for 5 min. K. Macaluso provided the expert laboratory L1 with DNA from five R. felis-positive (strain LSU) C. felis fleas [14].

at 94°C was followed by 40 cycles comprised of 30 sec. at 94°C, annealing at 58°C for 1 min.

The steps of the quantification PCR included initial denaturation at 94 °C for 2 min following which samples were subjected to 40 amplification cycles comprising of denaturation at 95 °C for 15 s, annealing and elongation of each gene for 60 s.

The reaction was performed in a Gene Amp PCR system 9700 thermo cycler (Applied Biosystems, USA) under the following conditions: initial denaturation at 94°C for 5 minutes followed by 35 amplification cycles comprising of 30 seconds denaturation at 94°C, 30 seconds annealing at 62°C, 60 seconds extension at 72°C, followed by a final extension step at 72°C for 7 minutes.

PCR conditions were: initial denaturation at 94°C for 5 minutes followed by 30 amplification cycles comprising of 30 seconds denaturation at 94°C, 30 seconds annealing at 58°C, 60 seconds extension at 72°C, followed by a final extension step at 72°C for 5 minutes.

The thermocycling conditions included an initial denaturation at 95 °C for 7 min, followed by 30 cycles comprising of a 30-s denaturation step at 95 °C, a 30-s annealing step at 55 °C, and a 4-min elongation step at 72 °C, and one final elongation step carried out at 72 °C for 10 min.

However, quantitative characterization of the underlying thermodynamic cycle comprised of protonation and conformational equilibria has remained an elusive goal.