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We do this by first detecting communities on the simplicial complex and then quantifying how many communities a cycle traverses as a function of its length.
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However, the appearance of harmonic peaks in limit cycle oscillations is sensitive to the shape of the limit cycle, for example, a circular limit cycle traversed at constant speed would not have harmonic peaks.
Inhibition of cell cycle traverse was confirmed by flow cytometric analysis.
- miR10b controls GBM cell and stem cell cycle traverse and is correlated with poor prognosis (http://tcga-data.nci.nih.gov/tcga).
Ezh2 may also promote cancer cell proliferation by modifying non-histone proteins to create methyl-degrons recognized by ubiquitin ligases that induce cell cycle traverse.
However, the use of replication inhibitors can also inhibit DNA polymerase-dependent DNA repair processes and can disrupt cycle traverse (Moore and Randall, 1987).
To study the cell cycle dependency of the effects of topotecan, single 1 h pulse exposures were used to minimise the extent of ongoing cycle traverse during the treatment period.
Cultures were exposed to vehicle or increasing concentrations of MF for 72 h and analysed for cell number and cell cycle traverse, and hypodiploid DNA content characteristic of apoptotic cell death.
To further investigate the mechanism of action of I'm-Yunity™ (PSP), we studied the effects of water extracts of I'm-Yunity™ (PSP) on growth, viability and cell cycle traverse using human HL-60 and U-937 leukemia cells.
The pulse exposure regimen was chosen so that the perturbing effects of the relatively unstable drug and the discreet responses of the cells could be resolved with the minimum of cycle traverse during the treatment period.
Consistent with previous observations, we confirmed that continuous exposures to topotecan led to slowing of cell cycle traverse for G1- and S-phase cells, with accumulation of cells at G2.
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