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The purified products were cycle-sequenced with Big Dye terminators (Applied Biosystems, Foster City, CA, USA), and the cycle sequence products were analysed with an automated sequencer (ABI Prism 310 Genetic Analyser).
Nucleotide sequences were determined with an Applied Biosystems 373 DNA sequencer using a BigDye Terminator v.3.1 Cycle Sequence Kit (Applied Biosystems).
The nucleotide sequence was determined using an Applied Biosystems 373 DNA sequencer with a BigDye Terminator v3.1 Cycle Sequence Kit (Applied Biosystems).
Nucleotide sequencing of the purified PCR products was performed using the ABI PRISM BigDye Terminator cycle sequence reaction kit and the automatic DNA sequencer ABI PRISM, model 377 (Applied Biosystems, Foster City, CA, USA), at the Unidad de Genómica, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid (UCM), Madrid, Spain.
Nucleotide sequences were determined by using the Terminator Cycle Sequence Kit version 3.1 and an ABI 3130 sequencer (both from Applied Biosytems, Boston, MA, USA).
Nucleotide sequences were determined with the terminator cycle sequence kit (version 3.1) and the ABI 3130 Avant sequencer (PerkinElmer Biosystems, Wellesley, MA, USA).
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Sequencing was performed using BigDye Terminator cycle sequencing (Applied Biosystems, Foster City, CA) on an ABI 377 DNA sequencer.
Single PCR products were analysed using the ABI 3130 Sequence Analyzer after standard Sanger-cycle-sequencing using BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Life Technologies, Darmstadt, Germany).
Samples were genotyped through Sanger sequencing using BigDye™ Terminator cycle sequencing kit (Applied Biosystems, USA).
PIK3CA C420R and E418K and GATA3 K358fs mutations were validated by cycle sequencing.
Sequencing was performed using a BigDye Terminator Cycle Sequencing Kit and an ABI PRISM 3130 Genetic Analyzer (Life Technologies).
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