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All mice were kept in a 12 h dark/light cycle room with humidity-control at a constant temperature of 25 °C.
Mice employed for the E11, and E12 time points were housed in a reverse light cycle room to facilitate dissections at midday for all samples.
In brief, rats (n = 12) were given access to one bottle of 5% sucrose and one bottle of water for three 24-hour-sessions per week (Mondays, Wednesdays and Fridays), 15 minutes after the lights went out in a reversed light/dark cycle room.
In brief, male C57BL/6 mice (5 6 weeks of age and 22±0.5g) were given access to one bottle of 20% ethanol and one bottle of water during a two-hour-period (1pm-3pm), Monday to Friday in a reverse light/dark cycle room.
All mice were housed in 12-hr light/dark cycle room with food and water provided ad libitum.
In climate-controlled rooms (22 ± 2°C), rats were housed in a reverse 12 h light/dark cycle room (lights off at 10 00 h).
Similar(48)
After multiple storage cycles (room temperature or 40 °C), the NZV coulombic efficiency (cell reversibility) exceeded 97% and the discharge capacity retention was >98%.
In the TNF-α (tumor necrosis factor alpha) and fasting experiments male mice C57BL/6 (Harlan, Italy) age between 10 and 12 weeks were housed in normal light-cycle room, maintained on standard rodent chow, and were allowed water and food ad libitum [ 15].
Four groups (6 animals per group) of female mice C57BL/6 (Harlan) age between 18 and 19 weeks were housed in normal light-cycle room, maintained on standard rodent chow (diet 3430, Provimi Kliba SA, Kaiseraugst, CH), and allowed water and food ad libitum.
Cells were lysed by three to four freeze cycles (in liquid nitrogen) and thaw cycles (room temperature).
Adult male SD rats (300 g–350 g) were purchased from the Animal Center of Tongji Medical College, HUST and housed in a 12 : 12 h light dark cycled room maintained at 22±2 °C with food and water ad libitum for 5 days prior to the experiments.
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