Morphine failed to induce detectable alteration in the cell cycle profile of viral infected cells.
Previous works had established that Inhibiting Aurora kinase obtained a polyploidal cell cycle profile [40 42].
Surprisingly, the cell cycle profile of HCT116 cells treated with 15 for 24 h showed an increase in tetraploid cells.
The cell cycle profile of transduced cells was analyzed as previously described [70].
Cell cycle profile was assessed by fluorescence-activated cell sorting as previously described [46].
Cell cycle profile was analysed with a Facscalibur apparatus (BD Biosciences).
Cell cycle profile distributions were determined with the Modfit LT 3.0 software.
Control experiments showed that the PolyLacO tag does not affect cell proliferation, cell cycle profile or Ig gene diversification.
We also analyzed the cell cycle profile by propidium iodide (PI) staining on day 3 and day 4.
To do so, we analyzed the cell cycle profile at various thymidine concentrations following after 12 h of treatment.
