Sentence examples for cycle of amplification from inspiring English sources

Moreover, by the combination of polymerase-catalyzed incorporation of lesion bases with UDG and Endo IV-assisted ERA, multiple cycle of amplification of the recognition event is achieved, enabling ultrasensitive detection of pathogenic bacteria.

The temperature cycle of amplification was as follows: the initial denaturation at a temperature of 94 °C for 2 min, and then successive denaturation (94 °C for 1 min), annealing (55 °C for 1 min), and extension (72 °C for 2 min).

The 1st cycle of amplification was conducted in 65 µl reaction mixture.

In fact, when amplification begins with a single molecule, an amplification that changes sequence would have to occur during the very first or second cycle of amplification to subsequently be detected via dideoxy-sequencing.

Each cycle of amplification was repeated 35 times.

(ii) In the second cycle of amplification, 3' and 5' ends varies more complicated.

The PCR cycling consisted of 40 cycles of amplification of the template DNA with primer annealing at 60 °C.

Cycling conditions were as above, but 35 cycles of amplification were used.

The thermal cycling profile for the telomere amplification will be 30 cycles of amplification at 95°C for 15 s and at 56°C for 60 s.

The amplification was performed at 94°C for 5 min, followed by 30 cycles of amplification (94°C for 1 min, 60°C for 1 min, and 72°C for 72 s).

After 5 min at 95°C, the PCR involved 35 cycles of amplification, each cycle comprising 30s at 95°C, 30s at 55°C, 30s at 72°C; and with a final extension step of 5 min at 72°C.