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One cycle of affinity maturation was performed.
The protein was readily enriched more than 8-fold to higher than 95% homogeneity by a single cycle of affinity chromatography in a Ni-chelating column (Support information Table S1).
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A population of AR cells was selected by repeated cycles of affinity cell sorting [37] using magnetic Dynabeads M-450 (DYNAL, NY) coated with IL-2R antibodies.
This also includes enrichment of high affinity and high specificity binding motifs by repetitive cycles of affinity selection and enzymatic amplification simultaneously.
CTGF containing fractions were pooled and subjected to a second cycle of heparin affinity chromatography.
The protein was further purified by a second cycle of nickel affinity chromatography using the same column.
As a whole, four cycles of selection on SM2-OVA, as well as one round of affinity maturation were performed.
Initial lead structures are, in general, optimized in terms of affinity using iterative cycles comprising synthesis, biological evaluation, computational methods, and structural analysis.
Hence, the difference formulation describes the number of cells Α k and Ω k in compartments that are associated with the affinities (3) a k = e − kp with k ∈ 0, 1, …, 127 While the quiescent cells in GE Α are fully characterized by their affinity a, in GE Ω the cellular state is given by the combination of affinity a and cell cycle position c.
And it means having lots of "hooks" that help to attach seekers to the church.Willow Creek has dozens of affinity groups for everyone from motor-cycle enthusiasts to weight-watchers.
After seven cycles of selection using affinity elution, 35%to45%5% of the input RNA was retained on the resin and could be eluted with free CoA.
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