When overexpressed, Mia1p-MutNES4-mCherry accumulated in the nucleus at all stages of cell cycle, mimicking localization of this protein expressed under its native promoter (Fig. 4E).
Our analysis revealed a down-regulation of the biological processes related to ubiquitin metabolism; cell signalling, with a particular enrichment for the NF- kB cascade; cell cycle; protein localization; regulation of gene expression (Table 6).
In this study, genes involved in ubiquitin metabolism, cell signalling (with a particular enrichment of NF- kB cascade), cell cycle, protein localization and regulation of gene expression were down-regulated, and genes involved in developmental processes and calcium ion transport were up-regulated.
In particular, the identification of two hypothetical proteins with cell-cycle dependent localization patterns, one of which is localized to a helix-like structure, indicate that dynamic localization and complex three-dimensional structures may be common in C. crescentus.
Centrosomal proteins often exhibit cell cycle-dependent localization to the organelle, with their levels changing during the cell cycle.
Synchronized cultures have been used to detect cell-cycle dependent localization of several structural and signaling proteins [2], [11], but the number of proteins whose localization changes through the cell cycle is not known.
The time-lapse movies of the FtsZ-mEos2- and FtsZ-GFP-expressing strains showed essentially the same cell-cycle dependent localization patterns of FtsZ at the midcell (Figure 1C).
It is conceivable that this cell cycle-dependent localization confines PRL access to substrates.
In the case of the BACH1 and CHAF1B proteins, we observed a cell cycle-dependent localization pattern.
This cell-cycle regulated localization is dependent on constant endocytosis and recycling through the TGN (Valdivia et al., 2002).
