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It is not clear how such shortened NA stalk regions influences virulence, but early steps of the viral replication cycle (binding, immune evasion, prevention of apoptosis) may be involved in addition to tuning of the HA/NA balance.
There was slight enrichment for proteins involved in RNA metabolism; mRNAs encoding proteins with RNA-binding domains included the cell cycle binding sequence protein CSBP2, DRBD14, DRBD17, DRBD18, RBP10, RBP26, ZC3H12, ZC3H41 and ZC3H46 and Tb927.11.3500.
For instance, the hub genes were enriched by up to 103 fold for the following GO clusters: cell cycle, binding (adenyl nucleotide binding, purine nucleoside binding, ATP binding, nucleotide binding, nucleoside binding), chromosome segregation and sister chromatid segregation, cytoskeleton and microtubule organisation, condensed chromosome, microtubule and motor activity and DNA replication.
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Cyclin-dependent kinases (Cdks) are molecules that facilitate the progression of the cell cycle by binding to cyclins and phosphorylating downstream proteins that assist in cell cycle propagation.
Geminin also limits the extent of DNA replication during the cell cycle by binding and inhibiting the essential replication factor Cdt1 [27].
Nonetheless, we wished to observe clear CTCF nucleolar localization, and so used a stage of the cell cycle when binding to nucleolar DNA is cytologically distinct.
Rb is known to arrest cells in the G1 phase of the cell cycle by binding to E2F transcription factors (Reviewed by [56], while free E2F factors are important for the induction of S phase entry.
LL carried out cell cycle analysis, binding affinity assay, co-immunoprecipitation experiment, Western blotting, and gene functional assays.
Membrane-associated molecules of hsc70 actively disassemble LAMP-2A into monomers to initiate a new cycle of binding and translocation [ 28].
In contrast CT-Xic1, which inhibits the cell cycle by binding PCNA, cannot rescue the loss of differentiated heart tissue seen with Xic1Mo.
Although the RPA complex is ubiquitously expressed throughout the cell cycle, its binding to ssDNA is largely restricted to cells undergoing DNA replication (8).
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