For cell cycle analysis, attached and floating U87-2M1 cells were collected 3 days following transduction for propidium iodide staining and analyzed on the LSRII flow cytometry analyzer (BD Biosciences, San Jose, CA, USA).
Stigma and its counterpart, inclusion, are reflected in actions and the cycle of attaching a pejorative subtext will only repeat with any euphemism for those with disabilities unless the underlying negative connotations are removed.
During the parasitic period of their life cycle, they attach to fish with their suction disk mouth ringed with sharp teeth and use their tongue to rasp through the scales and skin of a host to feed on its blood and body fluids (Hardisty and Potter 1971).
Traditionally, arrotini made their rounds on bicycles or motorcycles, which provided transportation as well as a means to turn the grinder's wheel, which attached to the cycle.
From the perspective of a single molecule, the velocity of actin over a pure myosin is related to the inverse of its attached lifetime (ton) – how long during each hydrolytic cycle myosin remains attached to actin.
The energetic cost of low-load contraction is thus minimised, due to the strain dependence of the chemo-mechanical steps of the ATPase cycle in the attached heads2,7.
Mandy Moore has been giving TV the ol' college try for a few pilot seasons now and in this latest cycle, she was attached to both an ABC comedy, "Pulling," and a CBS drama, "The Advocates".
Similarly, the cost of being in each Markov state is computed for the length of the Markov cycle and is attached to the state.
As a consequence, the "duty ratio" of the motor (i.e., the fraction of the catalytic cycle spent tightly attached to the track) approaches unity.
1-cycles always attach to individual vertices and are not regarded as feedback loops.
