The ecologic, topographic, and climatic diversity of Colombia gives rise to 23 distinct vegetative zones (33 ) and a spatially heterogeneous distribution of transmission cycles and intensity of ACL transmission (15 ).
The samples were sonicated for 25 min with the following parameters with a Covaris S2 system: burst 200, cycle 20%, and intensity 8.
The effects of the frequency of the light/dark cycle and light intensity on the microalgae cultivation were also investigated.
Effect of culture depth, rocking cycle, and light intensity to algal biomass production, pH change, and DO accumulation were investigated in this system.
Light (a mixture of cool white and GRO-LUX fluorescent light bulbs) was turned on and off in a 12∶12 hr cycle, and the intensity (reaching cultures in transparent bottles) was of ca. 250 300 µmol photons m−2 s−1.
A uniaxial accelerometer, the StepWatch™ Activity Monitor (Orthocare Innovations, Seattle, Washington, USA), was used to determine the volume (number of gait cycles per day) and intensity (gait cycles per minute) of walking ability.
The cDNA fragments were sheared using a Covaris E110 (Covaris, Woburn, MA, USA) for 75 sec under conditions "Duty cycle" of 20% and "Intensity" of 5, then the cDNA fraction with lengths of 200-250 bp was excised from 8% polyacrylamide gel electrophoresis (PAGE) for cDNA library construction using a paired-end sample prep kit (Illumina, San Diego, CA, USA).
Plates were run for 40 cycles and fluorescence intensity measured after every cycle.
Light was not supplemented or masked and so daylight cycles and light intensity followed those for Sydney, NSW (latitude -33.9° S).
All Arabidopsis thaliana lines used in this work were grown in a growth chamber at 24/21°C with 16/8-h day/night cycles and light intensity of 300 μmol/m−2 s−1 as previously described [ 7, 22].
